Cat. No.: 312 111
Amount: 100 µg
Price:
$415.00
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Cat. No. 312 111 | 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in TRIS. Then aliquot and store at -20°C until use. |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.', $event)" style="cursor: help;">ELISA: yes |
Clone | JP39 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 1618 from human Nestin (UniProt Id: P48681) |
Reactivity |
Reacts with: human (P48681), mouse (Q6P5H2). Other species not tested yet. |
Specificity | Specific for human nestin. |
Remarks |
Differences between clone JP 63 and clone JP39 in application or specificity not determined yet. |
Data sheet | 312_111.pdf |
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