Synaptic Systems

Cat. No.: 292 015

Amount: 50 µg

Price: $465.00

IHC

IHC-P

Background

Olig2 (also known as BHLHB1 and PRKCBP2) is a basic helix–loop–helix transcription factor characteristic of the oligodendroglial lineage and plays key roles in the specification and maturation of oligodendrocytes. Oligodendrocytes are glial cells that produce and maintain central nervous system myelin; Morphologically, oligodendrocyte nuclei are typically smaller and more oval than nuclei of other CNS cell types. (1-3).
In cancer, particularly in diffuse gliomas, Olig2 is widely used as a nuclear immunohistochemical marker of glial lineage. In diagnostic pathology, nuclear Olig2 immunohistochemistry is widely used as a glial lineage marker in diffuse gliomas: Olig2 is strongly expressed in oligodendrogliomas and is also detectable in a substantial proportion of astrocytomas and glioblastomas (GBM). Large cohorts established that Olig2 is highly expressed across diffuse gliomas while being largely restricted to oligodendroglia in normal brain, supporting its utility in routine practice (4, 5).

1 ) Mei F et al., J. Neurosci. (2013)
2 ) Chung SH et al., Cell Death Dis (2013)
3 ) Hafler BP et al., Proc. Natl. Acad. Sci. U.S.A. (2012)
4 ) Ligon KL et al., J Neuropathol Exp Neurol (2004)
5 ) Wesseling P et al., Acta Neuropathol (2015)

Protocols

IHC

IHC-P

Other products for this target

Cat. No. 292 015	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 2000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length mouse recombinant Olig2 (UniProt Id: Q9EQW6)
Reactivity	Reacts with: mouse (Q9EQW6), rat (G3V612), human (Q13516). Other species not tested yet. Predicted to cross-react with human (Q13516) due to high sequence homology.
Data sheet	292_015.pdf

Olig2 antibody - 292 015