EPS8 antibody - 288 003 K.O.

EPS8 is an actin binding regulatory protein

Rabbit polyclonal purified antibody

Cat. No.: 288 003

Amount: 50 µg

Price: $380.00

Cat. No. 288 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 648 to 821 from mouse EPS8 (UniProt Id: Q08509)
Reactivity	Reacts with: rat (F1M3L7), mouse (Q08509). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 288_003

Cat. No.: 288 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Actin dynamics are controlled by ensembles of actin-binding proteins. These processes include binding and/or sequestering of actin monomers, nucleation of actin filaments, capping or anticapping of barbed ends, and severing, bundling, and anchoring of F-actin (filamentous actin).
The epidermal growth factor receptor pathway substrate 8 (EPS 8) belongs to a family of proteins that is involved in the signal-transduction from Ras to Rac mediating actin remodeling. It contains a proline-rich domain, a phosphotyrosine binding (PTB) domain, an SH3-binding domain and directly caps barbed actin ends and cross-links actin filaments.

1 ) Cheng CY et al., Spermatogenesis (2011)
2 ) Hertzog M et al., PLoS Biol. (2010)
3 ) Menna E et al., PLoS Biol. (2009)
4 ) Sekerková G et al., Neuroscience (2007)
5 ) Roffers-Agarwal J et al., BMC Cell Biol. (2005)

Protocols

ICC

Fixed Cells

IHC

Standard Condition