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S100B - 287 108

A marker for mature astrocytes
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 287 108
Amount: 50 µg
Price: 415.00 €
Cat. No. 287 108 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 200 gallery  
Clone Rb86D7E4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity Reacts with: mouse (P50114), rat (P04631).
Other species not tested yet.
Specificity Specific for S100B, no cross-reactivity to S100A
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 86D7E4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 287_108.pdf
Cat. No.: 287 108
Quantity: 50 µg
Price: 415.00 €
Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca2+-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.