VDAC1 antibody - 279 003

VDAC1 is a pore forming transmembrane protein

Rabbit polyclonal purified antibody

Cat. No.: 279 003

Amount: 50 µg

Price: $380.00

Cat. No. 279 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 15 to 29 from mouse VDAC1 (UniProt Id: Q60932-1)
Reactivity	Reacts with: rat (Q9Z2L0), mouse (Q60932). Other species not tested yet.
Specificity	Due to sequence homologies, probably crossreacts with VDAC 2 and 3
Matching control protein/peptide	279-0P
Data sheet	Datasheet 279_003

Cat. No.: 279 003

Amount: 50 µg

Price: $380.00

Background

Voltage-dependent anion channels (VDACs), also known as porins, are integral membrane proteins which form a channel that allows diffusion of small hydrophilic molecules. Three closely related isoforms, VDAC 1, 2 and 3 have been described, so far.
VDACs have been originally identified as components of the outer mitochondrial membrane but they also occur in the plasma membrane of different cell types.

1 ) Kotake S et al., Cell Biol. Int. (2013)
2 ) Abu-Hamad S et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
3 ) Adachi M et al., Am. J. Physiol. Gastrointest. Liver Physiol. (2004)
4 ) Bahamonde MI et al., Pflugers Arch. (2003)
5 ) Narita M et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
6 ) De Pinto V et al., Biochim. Biophys. Acta (1987)
7 ) Colombini M et al., Ann. N. Y. Acad. Sci. (1980)

Protocols

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