TEV-cut site antibody - 265 003

TEV is a cysteine protease frequently used in biochemistry

Rabbit polyclonal purified antibody

Cat. No.: 265 003

Amount: 50 µg

Price: $380.00

Cat. No. 265 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 6 from TEV-cut site
Specificity	TEV-cut protease site is recognized with very strong preference.
Data sheet	Datasheet 265_003

Cat. No.: 265 003

Amount: 50 µg

Price: $380.00

ICC

Background

TEV is a highly specific cysteine protease derived from Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence (ENLYFQ(G/S)) and cleavage occurs between the Q and G/S residues leaving a free carboxy-terminus.

1 ) Sun C et al., Protein Expr. Purif. (2012)
2 ) Lundbäck AK et al., Anal. Biochem. (2008)
3 ) Phan J et al., J. Biol. Chem. (2002)
4 ) Kapust RB et al., Biochem. Biophys. Res. Commun. (2002)
5 ) Kapust RB et al., Protein Eng. (2001)

Protocols

ICC

Fixed Cells

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