Giantin antibody - 263 003BT

Giantin is specifically expressed in the Golgi complex

Rabbit polyclonal purified antibody

Cat. No.: 263 003BT

Amount: 50 µg

Price: $485.00

Cat. No. 263 003BT	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Immunogen	Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)
Reactivity	Reacts with: rat (G3V6A8), mouse. No signal: zebrafish. Other species not tested yet.
Data sheet	Datasheet 263_003bt

Cat. No.: 263 003BT

Amount: 50 µg

Price: $485.00

ICC

Background

In eukaryotic cells the Golgi complex consists of stacked cisternae. Giantin, also referred to as macrogolgin or p400, is a single pass membrane protein involved in tethering these cisternae by cross-bridge formation. Its large cytoplasmic N-terminus is involved in the docking of COPI vesicles via p115 to the Golgi membrane.
Systemic lupus erythematosus and Sjögren's syndrome correlate with autoimmune antibodies against Golgi-compartment proteins with giantin being the most common autoantigen.

1 ) Rosing M et al., Exp. Cell Res. (2007)
2 ) Nozawa K et al., Arthritis Res. Ther. (2004)
3 ) Alvarez C et al., J. Biol. Chem. (2001)
4 ) Sönnichsen B et al., J. Cell Biol. (1998)
5 ) Seelig HP et al., Mol. Cell. Biol. (1994)
6 ) Linstedt AD et al., Mol. Biol. Cell (1993)

Protocols

ICC

Fixed Cells

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