Synaptic Systems

Cat. No.: 260 006

Amount: 50 µg

Price: $385.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 260 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 189 to 204 from rat NET (UniProt Id: Q63380)
Reactivity	Reacts with: rat (Q63380), mouse (O55192). Other species not tested yet.
Matching control protein/peptide	260-0P
Data sheet	260_006.pdf

References for NET - 260 006

Genetic disruption of dopamine β-hydroxylase dysregulates innate responses to predator odor in mice.
Liu J, Lustberg DJ, Galvez A, Liles LC, McCann KE, Weinshenker D
bioRxiv : the preprint server for biology (2023) : . 260 006 IHC; tested species: mouse

Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes.
Fischer T, Prey J, Eschholz L, Rotermund N, Lohr C
Frontiers in cellular neuroscience (2021) 15: 639754. 260 006 IHC; tested species: mouse

Immunohistochemical analysis of the mouse celiac ganglion: An integrative relay station of the peripheral nervous system.
Kaestner CL, Smith EH, Peirce SG, Hoover DB
The Journal of comparative neurology (2019) : . 260 006 IHC; tested species: mouse

Cat. No.: 260 006

Amount: 50 µg

Price: $385.00

IHC

Background

The norepinephrine (NE) transporter (NET), also referred to as Sodium-dependent noradrenaline transporter, is expressed on the plasma membranes of noradrenergic neurons. It is responsible for the inactivation of synaptically released NE and is a target for many antidepressants and psychostimulants.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Robertson SD et al., J. Neurosci. (2010)
2 ) Li Z et al., J. Neurosci. (2010)
3 ) Guiard BP et al., Mol. Pharmacol. (2008)
4 ) Schroeter S et al., J. Comp. Neurol. (2000)
5 ) Kuczenski R et al., J. Neurosci. (1995)

Protocols

IHC

OTHER

Preabsorbtion

Other products for this target

NET antibody - 260 006

References for NET - 260 006