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Chromogranin A antibody - 259 003 K.O.

Chromogranin A is expressed at high levels in the neuroendocrine system
Rabbit polyclonal purified antibody
Cat. No.: 259 003
Amount: 50 µg
Price: $455.00
Cat. No. 259 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 2000 gallery  
Immunogen Recombinant protein corresponding to AA 348 to 463 from mouse Chromogranin A (UniProt Id: P26339)
Reactivity Reacts with: rat (P10354), mouse (P26339), human (P10645).
Other species not tested yet.
Specificity K.O. validated PubMed: 29178418
Matching control protein/peptide 259-0P
Data sheet 259_003.pdf

References for Chromogranin A - 259 003

Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 259 003 WB, ICC; tested species: mouse
Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B.
Dominguez N, van Weering JRT, Borges R, Toonen RFG, Verhage M
Journal of neurochemistry (2018) 1443: 241-254. 259 003 WB; KO verified; tested species: mouse
Distinct Alterations in Dendritic Spine Morphology in the Absence of β-Neurexins.
Mohrmann L, Seebach J, Missler M, Rohlmann A
International journal of molecular sciences (2024) 252: . 259 003 ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 259 003 ICC; tested species: mouse
Prion protein conversion at two distinct cellular sites precedes fibrillisation.
Ribes JM, Patel MP, Halim HA, Berretta A, Tooze SA, Klöhn PC
Nature communications (2023) 141: 8354. 259 003 ICC; tested species: mouse
Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 003 ICC; tested species: mouse
Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 259 003 WB, ICC; tested species: mouse
Deletion of β-Neurexins in Mice Alters the Distribution of Dense-Core Vesicles in Presynapses of Hippocampal and Cerebellar Neurons.
Ferdos S, Brockhaus J, Missler M, Rohlmann A
Frontiers in neuroanatomy (2021) 15: 757017. 259 003 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 259 003 ICC; tested species: rat
Pool size estimations for dense-core vesicles in mammalian CNS neurons.
Persoon CM, Moro A, Nassal JP, Farina M, Broeke JH, Arora S, Dominguez N, van Weering JR, Toonen RF, Verhage M
The EMBO journal (2018) : . 259 003 IHC; tested species: mouse
Cat. No.: 259 003
Amount: 50 µg
Price: $455.00
Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 259 003 WB, ICC; tested species: mouse
Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B.
Dominguez N, van Weering JRT, Borges R, Toonen RFG, Verhage M
Journal of neurochemistry (2018) 1443: 241-254. 259 003 WB; KO verified; tested species: mouse
Distinct Alterations in Dendritic Spine Morphology in the Absence of β-Neurexins.
Mohrmann L, Seebach J, Missler M, Rohlmann A
International journal of molecular sciences (2024) 252: . 259 003 ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 259 003 ICC; tested species: mouse
Prion protein conversion at two distinct cellular sites precedes fibrillisation.
Ribes JM, Patel MP, Halim HA, Berretta A, Tooze SA, Klöhn PC
Nature communications (2023) 141: 8354. 259 003 ICC; tested species: mouse
Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.
Nassal JP, Murphy FH, Toonen RF, Verhage M
eLife (2022) 11: . 259 003 ICC; tested species: mouse
Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 259 003 WB, ICC; tested species: mouse
Deletion of β-Neurexins in Mice Alters the Distribution of Dense-Core Vesicles in Presynapses of Hippocampal and Cerebellar Neurons.
Ferdos S, Brockhaus J, Missler M, Rohlmann A
Frontiers in neuroanatomy (2021) 15: 757017. 259 003 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 259 003 ICC; tested species: rat
Pool size estimations for dense-core vesicles in mammalian CNS neurons.
Persoon CM, Moro A, Nassal JP, Farina M, Broeke JH, Arora S, Dominguez N, van Weering JR, Toonen RF, Verhage M
The EMBO journal (2018) : . 259 003 IHC; tested species: mouse
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WB
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IHC
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IHC-P
IHC-P
IHC-P
Background
Chromogranin A (CgA) and B (CgB) are members of a family of acidic proteins stored and released throughout the neuroendocrine system. The large dense core vesicle associated proteins have multiple functions in neurons and neuroendocrine cells. They are differentially processed in different tissues.
Chromogranin A (CgA) is the precursor for the bioactive peptides pancreastatin, vasostatins, catestatin, β-granin and WE-14.
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