Cannabinoid receptor CB1-R antibody - 258 308 K.O.

CB1-R is a G-protein coupled receptor responsive to cannabinoids

Guinea pig monoclonal recombinant IgG

Cat. No.: 258 308

Amount: 50 µg

Price: $420.00

Cat. No. 258 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp289C1
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat CB1-R (UniProt Id: P20272)
Reactivity	Reacts with: rat (P20272), mouse (P47746), human (P21554). Other species not tested yet.
Specificity	K.O. validated
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 289C1. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. RUO: For this product an additional Limited Use Label License (LULL) for internal Research Use Only (RUO) from Life Technologies Corporation applies. The recombinant proteins/antibodies you will receive/have received from Synaptic Systems GmbH (“Materials“) were provided or produced under a license between Synaptic Systems GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies“) through the use of Life Technologies proprietary cell lines and expression products. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit, up to and including pharmacokinetic and toxicology studies required to meet the regulations for filing an IND. IND submissions, first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial purposes or applications of any kind, including, without limitation, manufacturing or quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008, United States.
Data sheet	Datasheet 258_308

Cat. No.: 258 308

Amount: 50 µg

Price: $420.00

Indirect immunostaining of a mouse hippocampus section for CB1-R (red)

ICC
(1 img)

IHC
(3 img)

IHC-P (FFPE)
(2 img)

Background

The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.

1 ) Osborne KD et al., ASN Neuro (2009)
2 ) Niehaus JL et al., Mol. Pharmacol. (2007)
3 ) Kawamura Y et al., J. Neurosci. (2006)
4 ) Shire D et al., J. Biol. Chem. (1995)
5 ) Matsuda LA et al., Nature (1990)
6 ) Howlett AC et al., Mol. Pharmacol. (1985)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target