Cre-Recombinase antibody - 257 008

Cre-recombinase is used for conditional or tissue specific knockouts

Rabbit monoclonal recombinant IgG

Cat. No.: 257 008

Amount: 50 µg

Price: $420.00

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Cat. No. 257 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb271B7
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant Cre-Recombinase (UniProt Id: P06956)
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone SY-271B7. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Data Sheet 257_008 Safety Data Sheet (SDS)

Cat. No.: 257 008

Amount: 50 µg

Price: $420.00

Immunofluorescence image of a Nex-Cre mouse hippocampus section stained for Cre-Recombinase (red)

ICC
(1 img)

IHC
(2 img)

IHC-P (FFPE)
(1 img)

Background

Cre-Recombinase is a site-specific DNA recombinase derived from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites, enabling precise deletion, inversion, or activation of DNA sequences. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state [1].
In experimental biology, Cre is commonly expressed under tissue-, cell-, or temporally specific promoters, allowing conditional manipulation of genes in transgenic and knockout mouse models [2, 3]. Inducible variants such as Cre-ERT are fused to a modified estrogen receptor domain and can be activated by tamoxifen, providing temporal control of recombination [4].

1 ) Lanza AM et al., Biotechnol J (2012)
2 ) Nagy A et al., Genesis (2000)
3 ) Brault V et al., Handb Exp Pharmacol (2007)
4 ) Feil R et al., Proc Natl Acad Sci U S A (1996)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

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