|Cat. No. 251 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)|
Epitop: AA 2 to 14 from mouse β-Actin (UniProt Id: P60710)
Reacts with: rat (P60711), mouse (P60710), human (P60709), zebrafish.
Other species not tested yet.
|Specificity||May cross-react to α- and γ-actin due to sequence homology.|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 130B4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.