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beta-Actin antibody - 251 003

beta-Actin is a major constituent of the cytoskeleton

 

  independently validated
  by Science Exchange initiative
Polyclonal rabbit purified antibody
Cat. No.: 251 003
Amount: 100 µg
Price: $370.00
Cat. No. 251 003 100 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity Reacts with: rat (P60711), mouse (P60710), chicken, zebrafish.
Other species not tested yet.
Specificity May cross-react to α- and γ-actin due to sequence homology.
Documents Science Exchange validation report
Remarks

ICC: Methanol or mild PFA fixation (2%).

Data sheet 251_003.pdf

References for beta-Actin - 251 003

Deletion of hippocampal Glucocorticoid receptors unveils sex-biased microRNA expression and neuronal morphology alterations in mice.
Tejos-Bravo M, Oakley RH, Whirledge SD, Corrales WA, Silva JP, García-Rojo G, Toledo J, Sanchez W, Román-Albasini L, Aliaga E, Aguayo F, et al.
Neurobiology of stress (2021) 14: 100306. 251 003 WB; tested species: mouse
The Synaptic Vesicle Priming Protein CAPS-1 Shapes the Adaptation of Sensory Evoked Responses in Mouse Visual Cortex.
Nestvogel DB, Merino RM, Leon-Pinzon C, Schottdorf M, Lee C, Imig C, Brose N, Rhee JS
Cell reports (2020) 3010: 3261-3269.e4. 251 003 WB; tested species: mouse
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, et al.
Nature communications (2020) 111: 2729. 251 003 WB; tested species: rat
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 251 003 WB; tested species: human
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 251 003 WB; tested species: mouse
The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
Baron J, Blex C, Rohrbeck A, Rachakonda SK, Birnbaumer L, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2013) 1246: 782-94. 251 003 WB
Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.
Whitemarsh RC, Strathman MJ, Chase LG, Stankewicz C, Tepp WH, Johnson EA, Pellett S
Toxicological sciences : an official journal of the Society of Toxicology (2012) 1262: 426-35. 251 003 WB
Cat. No.: 251 003
Quantity: 100 µg
Price: $370.00
Deletion of hippocampal Glucocorticoid receptors unveils sex-biased microRNA expression and neuronal morphology alterations in mice.
Tejos-Bravo M, Oakley RH, Whirledge SD, Corrales WA, Silva JP, García-Rojo G, Toledo J, Sanchez W, Román-Albasini L, Aliaga E, Aguayo F, et al.
Neurobiology of stress (2021) 14: 100306. 251 003 WB; tested species: mouse
The Synaptic Vesicle Priming Protein CAPS-1 Shapes the Adaptation of Sensory Evoked Responses in Mouse Visual Cortex.
Nestvogel DB, Merino RM, Leon-Pinzon C, Schottdorf M, Lee C, Imig C, Brose N, Rhee JS
Cell reports (2020) 3010: 3261-3269.e4. 251 003 WB; tested species: mouse
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, et al.
Nature communications (2020) 111: 2729. 251 003 WB; tested species: rat
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 251 003 WB; tested species: human
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 251 003 WB; tested species: mouse
The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
Baron J, Blex C, Rohrbeck A, Rachakonda SK, Birnbaumer L, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2013) 1246: 782-94. 251 003 WB
Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.
Whitemarsh RC, Strathman MJ, Chase LG, Stankewicz C, Tepp WH, Johnson EA, Pellett S
Toxicological sciences : an official journal of the Society of Toxicology (2012) 1262: 426-35. 251 003 WB
Background
The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.