VAP-A antibody - 249 003

VAP-A is a SNARE binding protein

Rabbit polyclonal purified antibody

Cat. No.: 249 003

Amount: 50 µg

Price: $460.00

Cat. No. 249 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 223 from human VAP-A (UniProt Id: Q9P0L0)
Reactivity	Reacts with: human (Q9P0L0), rat (Q9Z270), mouse (Q9WV55). Other species not tested yet.
Matching control protein/peptide	249-0P
Data sheet	Datasheet 249_003

Cat. No.: 249 003

Amount: 50 µg

Price: $460.00

ICC

Background

The VAMP-associated protein A VAP-A, also referred to as VAP-33, is a ubiquitously expressed type IV membrane protein. It is composed of an N-terminal domain with an immunoglobulin-like b-sheet, a central coiled-coiled domain and a C-terminal transmembrane domain. The protein, which may be involved in vesicle trafficking, is located to the endoplasmic reticulum (ER) and ER/Golgi intermediate compartment (ERGIC). VAP-A has been shown to bind to dfifferent v- and t-SNARE proteins.
Two other isoforms, VAP-B and VAP-C, have been described in the literature so far.

1 ) Tuuf J et al., Biochem. Biophys. Res. Commun. (2009)
2 ) Ettayebi K et al., J. Virol. (2003)
3 ) Wyles JP et al., J. Biol. Chem. (2002)
4 ) Weir ML et al., Biochem. Biophys. Res. Commun. (2001)

Protocols

ICC

Fixed Cells

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