BASP1 antibody - 246 003

BASP1 is a neuronal protein locating to axonal membranes

Rabbit polyclonal purified antibody

Cat. No.: 246 003

Amount: 50 µg

Price: $380.00

Cat. No. 246 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 220 from rat BASP1 (UniProt Id: Q05175)
Reactivity	Reacts with: rat (Q05175), mouse. Other species not tested yet.
Remarks	WB: Due to its acidic pH, this protein migrates at higher molecular weight than theoretically calculated.
Data sheet	Datasheet 246_003

Cat. No.: 246 003

Amount: 50 µg

Price: $380.00

ICC

IHC-P (FFPE)

Background

The brain acid soluble protein 1 (BASP 1) or neuronal axonal membrane protein (NAP-22) is a Ca²⁺-dependent calmodulin-binding protein that is specifically expressed in neuronal tissue.
BASP 1 has several phosphorylation sites, undergoes N-terminal myristoylation and has been shown to be involved in intracellular signalling processes modulating neurite outgrowth.

1 ) Ostroumova OS et al., FEBS J. (2011)
2 ) Zakharov VV et al., J. Struct. Biol. (2010)
3 ) Green LM et al., Nucleic Acids Res. (2009)
4 ) Korshunova I et al., J. Neurosci. Res. (2008)
5 ) Mosevitsky MI et al., Int. Rev. Cytol. (2005)
6 ) Zakharov VV et al., Biochim. Biophys. Acta (2003)
7 ) Mosevitsky MI et al., Biochimie (1997)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)