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HA-tag antibody - 245 011

The HA-Tag is a widely used epitope tag
Mouse monoclonal purified IgG
Cat. No.: 245 011
Amount: 200 µg
Price: $415.00
Cat. No. 245 011 200 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks)
IP: yes
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Clone 12CA5
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide (YPYDVPDYA) corresponding to residues AA 98-106 from human hemaglutinin
Remarks

WB: This antibody tends to produce unspecific background bands around 90-100 kDa. Therefore, we recommend 245 003 for westernblot experiments.

Data sheet 245_011.pdf
Cat. No.: 245 011
Amount: 200 µg
Price: $415.00
Background
The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.