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HA-tag antibody - 245 003C3

The HA-Tag is a widely used epitope tag
Rabbit polyclonal purified antibody
Cat. No.: 245 003C3
Amount: 50 µg
Price: $475.00
Cat. No. 245 003C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label Oyster 550
Immunogen Synthetic peptide corresponding to AA 98 to 108 from human HA-tag
Specificity Specific for HA-tag, hemaglutinin.
Matching control protein/peptide 245-0P
Data sheet 245_003c3.pdf

References for HA-tag - 245 003C3

TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
Yoon S, Parnell E, Penzes P
Cell reports (2020) 318: 107685. 245 003C3 ICC; tested species: mouse
Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development.
Yoon S, Parnell E, Kasherman M, Forrest MP, Myczek K, Premarathne S, Sanchez Vega MC, Piper M, Burne THJ, Jolly LA, Wood SA, et al.
Neuron (2020) 1053: 506-521.e7. 245 003C3 ICC; tested species: mouse
Cat. No.: 245 003C3
Amount: 50 µg
Price: $475.00
TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
Yoon S, Parnell E, Penzes P
Cell reports (2020) 318: 107685. 245 003C3 ICC; tested species: mouse
Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development.
Yoon S, Parnell E, Kasherman M, Forrest MP, Myczek K, Premarathne S, Sanchez Vega MC, Piper M, Burne THJ, Jolly LA, Wood SA, et al.
Neuron (2020) 1053: 506-521.e7. 245 003C3 ICC; tested species: mouse
Background
The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.
Protocols