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GluN2A/B antibody - 244 003

GluN2A and B are subunits of ionotropic NMDA type glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 244 003
Amount: 50 µg
Price: $455.00
Cat. No. 244 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 250 gallery  
IHC: not tested yet
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 1448 to 1464 from mouse GluN2A (UniProt Id: P35436)
Reactivity Reacts with: rat (Q00959, Q00960), mouse (P35436, Q01097).
Other species not tested yet.
Specificity Cross-reacts to GluN 2B due to sequence homology.
Matching control protein/peptide 244-0P
Remarks

IP: For most effective IP use the denaturing SDS based solubilization protocol. Consider that protein-protein interactions may be affected.

Data sheet 244_003.pdf

References for GluN2A/B - 244 003

Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum.
Chipman PH, Fung CCA, Pazo Fernandez A, Sawant A, Tedoldi A, Kawai A, Ghimire Gautam S, Kurosawa M, Abe M, Sakimura K, Fukai T, et al.
eLife (2021) 10: . 244 003 WB; tested species: mouse
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 003 WB, ICC; tested species: mouse
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 003 WB, ICC; tested species: mouse
Cat. No.: 244 003
Amount: 50 µg
Price: $455.00
Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum.
Chipman PH, Fung CCA, Pazo Fernandez A, Sawant A, Tedoldi A, Kawai A, Ghimire Gautam S, Kurosawa M, Abe M, Sakimura K, Fukai T, et al.
eLife (2021) 10: . 244 003 WB; tested species: mouse
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 003 WB, ICC; tested species: mouse
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 244 003 WB, ICC; tested species: mouse
Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN 1 and GluN 2 A-D. GluN 1 is the most important as it is required for activity. NMDA-receptors allow Ca2+ influx and are thought to trigger Ca2+ dependent postsynaptic processes involved in long term potentiation and depression.