|Cat. No. 244 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 250 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 1448 to 1464 from mouse GluN2A (UniProt Id: P35436)|
Reacts with: rat (Q00959, Q00960), mouse (P35436, Q01097).
Other species not tested yet.
|Specificity||Cross-reacts to GluN 2B due to sequence homology.|
|Matching control protein/peptide||244-0P|
IP: For most effective IP use the denaturing SDS based solubilization protocol. Consider that protein-protein interactions may be affected.
GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN 1 and GluN 2 A-D. GluN 1 is the most important as it is required for activity. NMDA-receptors allow Ca2+ influx and are thought to trigger Ca2+ dependent postsynaptic processes involved in long term potentiation and depression.