Synaptic Systems

Cat. No.: 235 003

Amount: 50 µg

Price: $380.00

Cat. No. 235 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 495 to 509 from human GLUT4 (UniProt Id: P14672)
Reactivity	Reacts with: human (P14672), rat (P19357), mouse (P14142), pig. Other species not tested yet.
Matching control protein/peptide	235-0P
Data sheet	Datasheet 235_003

References for GLUT4 - 235 003

EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes.
Koester AM, Geiser A, Laidlaw KME, Morris S, Cutiongco MFA, Stirrat L, Gadegaard N, Boles E, Black HL, Bryant NJ, Gould GW, et al.
Bioscience reports (2022) 427: . 235 003 WB; tested species: mouse

Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 235 003 WB

AMP-activated protein kinase is activated in adipose tissue of individuals with type 2 diabetes treated with metformin: a randomised glycaemia-controlled crossover study.
Boyle JG, Logan PJ, Jones GC, Small M, Sattar N, Connell JM, Cleland SJ, Salt IP
Diabetologia (2011) 547: 1799-809. 235 003 WB

CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.
Camus SM, Camus MD, Figueras-Novoa C, Boncompain G, Sadacca LA, Esk C, Bigot A, Gould GW, Kioumourtzoglou D, Perez F, Bryant NJ, et al.
The Journal of cell biology (2020) 2191: . 235 003 IHC; tested species: human

Cat. No.: 235 003

Amount: 50 µg

Price: $380.00

IHC

IHC-P (FFPE)

Background

Insulin stimulates glucose transport into muscle and fat cells by the redistribution of the glucose transporters 1 and 4 (GLUT 1 and GLUT 4) from intracellular membrane compartments to the cell surface via GLUT carrying vesicles.
Formation of soluble SNARE complexes mediate the docking and fusion of GLUT 4-containing vesicles with the plasma membrane.

1 ) Fukuda N et al., Diabetes (2009)
2 ) Li Y et al., Biochem. Biophys. Res. Commun. (2007)
3 ) Foster LJ et al., Am. J. Physiol., Cell Physiol. (2000)
4 ) Thurmond DC et al., J. Biol. Chem. (1998)
5 ) Haney PM et al., J. Cell Biol. (1991)

Protocols

IHC

IHC-P (FFPE)

Other products for this target

GLUT4 antibody - 235 003

References for GLUT4 - 235 003