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IBA1 antibody - 234 009

IBA1 is a marker for macrophages and microglia
Chicken monoclonal recombinant IgY
Cat. No.: 234 009
Amount: 50 µg
Price: $415.00
Cat. No. 234 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
ExM: 1 : 100
Clone Ch311H9
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008), ape.
Other species not tested yet.
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 234_009.pdf

References for IBA1 - 234 009

Meningeal macrophages protect against viral neuroinfection.
Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, Bardin Q, et al.
Immunity (2022) 5511: 2103-2117.e10. 234 009 IHC; tested species: monkey
Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring.
Smith CJ, Lintz T, Clark MJ, Malacon KE, Abiad A, Constantino NJ, Kim VJ, Jo YC, Alonso-Caraballo Y, Bilbo SD, Chartoff EH, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) 4710: 1755-1763. 234 009 IHC; tested species: rat
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 ExM, IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 ExM, IHC-P; tested species: human
Cat. No.: 234 009
Amount: 50 µg
Price: $415.00
Meningeal macrophages protect against viral neuroinfection.
Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, Bardin Q, et al.
Immunity (2022) 5511: 2103-2117.e10. 234 009 IHC; tested species: monkey
Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring.
Smith CJ, Lintz T, Clark MJ, Malacon KE, Abiad A, Constantino NJ, Kim VJ, Jo YC, Alonso-Caraballo Y, Bilbo SD, Chartoff EH, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) 4710: 1755-1763. 234 009 IHC; tested species: rat
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 ExM, IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 ExM, IHC-P; tested species: human
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).