Synaptic Systems

Cat. No.: 234 009

Amount: 50 µg

Price: $430.00

Cat. No. 234 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 200 up to 1 : 1000 gallery ExM: 1 : 100 Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Clone	Ch311H9
Subtype	IgY (λ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity	Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008), ape. Other species not tested yet.
Matching control protein/peptide	234-0P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	234_009.pdf

References for IBA1 - 234 009

Primary cortical cell tri-culture to study effects of amyloid-β on microglia function and neuroinflammatory response.
Kim H, Le B, Goshi N, Zhu K, Grodzki AC, Lein PJ, Zhao M, Seker E
Journal of Alzheimer's disease : JAD (2024) : 13872877241291142. 234 009 ICC; tested species: rat

Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes.
Wogram E, Sümpelmann F, Dong W, Rawat E, Fernández Maestre I, Fu D, Braswell B, Khalil A, Buescher JM, Mittler G, Borner GHH, et al.
Immunity (2024) 579: 2216-2231.e11. 234 009 ICC; tested species: human

Layer-specific molecular signatures of colon anastomotic healing and leakage in mice.
Sengul H, Bantavi V, Gloeck L, Li Yim AYF, Leven P, Efferz P, Schneiker B, Lysson M, De Jonge WJ, Wehner S
Molecular medicine (Cambridge, Mass.) (2025) 311: 124. 234 009 IHC; tested species: mouse

Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 009 IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 234 009 IHC; tested species: mouse

T cell-mediated microglial activation triggers myelin pathology in a mouse model of amyloidosis.
Kedia S, Ji H, Feng R, Androvic P, Spieth L, Liu L, Franz J, Zdiarstek H, Anderson KP, Kaboglu C, Liu Q, et al.
Nature neuroscience (2024) 278: 1468-1474. 234 009 IHC; tested species: mouse

The Abca7V1613M variant reduces Aβ generation, plaque load, and neuronal damage.
Butler CA, Mendoza Arvilla A, Milinkeviciute G, Da Cunha C, Kawauchi S, Rezaie N, Liang HY, Javonillo D, Thach A, Wang S, Collins S, et al.
Alzheimer's & dementia : the journal of the Alzheimer's Association (2024) 207: 4914-4934. 234 009 IHC; tested species: mouse

New AAV9 engineered variants with enhanced neurotropism and reduced liver off-targeting in mice and marmosets.
Giannelli SG, Luoni M, Iannielli A, Middeldorp J, Philippens I, Bido S, Körbelin J, Broccoli V
iScience (2024) 275: 109777. 234 009 IHC; tested species: mouse,marmoset

Modulation of central synapse remodeling after remote peripheral injuries by the CCL2-CCR2 axis and microglia.
Rotterman TM, Haley-Johnson Z, Pottorf TS, Chopra T, Chang E, Zhang S, McCallum WM, Fisher S, Franklin H, Alvarez M, Cope TC, et al.
Cell reports (2024) 432: 113776. 234 009 IHC; tested species: mouse

CCR2 restricts IFN-γ production by hippocampal CD8 TRM cells that impair learning and memory during recovery from WNV encephalitis.
Ai S, Arutyunov A, Liu J, Hill JD, Jiang X, Klein RS
Journal of neuroinflammation (2024) 211: 330. 234 009 IHC; tested species: mouse

Increased inflammation in older high-pressure glaucoma mice.
Reinehr S, Rahim Pamuk M, Fuchshofer R, Burkhard Dick H, Joachim SC
Neurobiology of aging (2024) 145: 55-64. 234 009 IHC; tested species: mouse

Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation.
Tegowski M, Prater AK, Holley CL, Meyer KD
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse

Chronic glial activation and behavioral alterations induced by acute/subacute pioglitazone treatment in a mouse model of traumatic brain injury.
Estrella LD, Manganaro JE, Sheldon L, Roland N, Snyder AD, George JW, Emanuel K, Lamberty BG, Stauch KL
Brain, behavior, and immunity (2024) 123: 64-80. 234 009 IHC; tested species: mouse

Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model.
Deppe L, Mueller-Buehl AM, Tsai T, Erb C, Dick HB, Joachim SC
Journal of personalized medicine (2024) 144: . 234 009 IHC; tested species: pig

Microglia maintain structural integrity during fetal brain morphogenesis.
Lawrence AR, Canzi A, Bridlance C, Olivié N, Lansonneur C, Catale C, Pizzamiglio L, Kloeckner B, Silvin A, Munro DAD, Fortoul A, et al.
Cell (2024) : . 234 009 IHC; tested species: mouse

Microglia regulate sleep through calcium-dependent modulation of norepinephrine transmission.
Ma C, Li B, Silverman D, Ding X, Li A, Xiao C, Huang G, Worden K, Muroy S, Chen W, Xu Z, et al.
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse

Lipofuscin-like autofluorescence within microglia and its impact on studying microglial engulfment.
Stillman JM, Mendes Lopes F, Lin JP, Hu K, Reich DS, Schafer DP
Nature communications (2023) 141: 7060. 234 009 IHC; tested species: mouse

Activation of β2-Adrenergic Receptors in Microglia Alleviates Neuropathic Hypersensitivity in Mice.
Damo E, Agarwal A, Simonetti M
Cells (2023) 122: . 234 009 IHC; tested species: mouse

HSP27 induced glaucomatous damage in mice of young and advanced age.
Erb C, Reinehr S, Theiss C, Dick HB, Joachim SC
Frontiers in cellular neuroscience (2023) 17: 1257297. 234 009 IHC; tested species: mouse

Control of hippocampal synaptic plasticity by microglia-dendrite interactions depends on genetic context in mouse models of Alzheimer's disease.
Heuer SE, Keezer KJ, Hewes AA, Onos KD, Graham KC, Howell GR, Bloss EB
Alzheimer's & dementia : the journal of the Alzheimer's Association (2023) : . 234 009 IHC; tested species: mouse

Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury.
Androvic P, Schifferer M, Perez Anderson K, Cantuti-Castelvetri L, Jiang H, Ji H, Liu L, Gouna G, Berghoff SA, Besson-Girard S, Knoferle J, et al.
Nature communications (2023) 141: 4115. 234 009 IHC; tested species: mouse

Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring.
Smith CJ, Lintz T, Clark MJ, Malacon KE, Abiad A, Constantino NJ, Kim VJ, Jo YC, Alonso-Caraballo Y, Bilbo SD, Chartoff EH, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) 4710: 1755-1763. 234 009 IHC; tested species: rat

Glutamate Metabotropic Receptor Type 3 (mGlu3) Localization in the Rat Prelimbic Medial Prefrontal Cortex.
Woo E, Datta D, Arnsten AFT
Frontiers in neuroanatomy (2022) 16: 849937. 234 009 IHC; tested species: rat

Activation of GPR183 by 7α,25-Dihydroxycholesterol Induces Behavioral Hypersensitivity through Mitogen-Activated Protein Kinase and Nuclear Factor-κB.
Braden K, Campolo M, Li Y, Chen Z, Doyle TM, Giancotti LA, Esposito E, Zhang J, Cuzzocrea S, Arnatt CK, Salvemini D, et al.
The Journal of pharmacology and experimental therapeutics (2022) 3832: 172-181. 234 009 IHC; tested species: mouse

The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 234 009 IHC; tested species: mouse

Meningeal macrophages protect against viral neuroinfection.
Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, Bardin Q, et al.
Immunity (2022) 5511: 2103-2117.e10. 234 009 IHC; tested species: monkey

Analgesic candidate adenosine A 3 receptors are expressed by perineuronal peripheral macrophages in human dorsal root ganglion and spinal cord microglia.
Sapio MR, Staedtler ES, King DM, Maric D, Jahanipour J, Ghetti A, Jacobson KA, Mannes AJ, Iadarola MJ
Pain (2024) 16510: 2323-2343. 234 009 IHC-P; tested species: human

Tau Accumulation Induces Microglial State Alterations in Alzheimer's Disease Model Mice.
Nagata K, Hashimoto S, Joho D, Fujioka R, Matsuba Y, Sekiguchi M, Mihira N, Motooka D, Liu YC, Okuzaki D, Kikuchi M, et al.
eNeuro (2024) 1112: . 234 009 IHC-P; tested species: mouse

Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 IHC-P, ExM; tested species: human

Single-cell image analysis reveals over-expression of organic anion transporting polypeptides (OATPs) in human glioblastoma tissue.
Cooper E, Woolf Z, Swanson MEV, Correia J, Schweder P, Mee E, Heppner P, Turner C, Faull RLM, Scotter EL, Denny WA, et al.
Neuro-oncology advances (2022) 41: vdac166. 234 009 IHC-P; tested species: human

Cat. No.: 234 009

Amount: 50 µg

Price: $430.00

Activation of β2-Adrenergic Receptors in Microglia Alleviates Neuropathic Hypersensitivity in Mice.
Damo E, Agarwal A, Simonetti M
Cells (2023) 122: . 234 009 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).

1 ) Köhler C et al., Cell Tissue Res. (2007)
2 ) Deininger MH et al., Acta Neuropathol. (2000)
3 ) Gehrmann J et al., Brain Res. Brain Res. Rev. (1995)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

IBA1 antibody - 234 009

References for IBA1 - 234 009