Home|||||||Technical Support|

IBA1 antibody - 234 008

IBA1 is a marker for macrophages and microglia
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 234 008
Amount: 50 µg
Price: $415.00
Cat. No. 234 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone Rb311H9
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 234_008.pdf
Cat. No.: 234 008
Quantity: 50 µg
Price: $415.00
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).