SV31 antibody - 228 002

SV31 is an integral synaptic vesicle membrane protein

Rabbit polyclonal antiserum

Cat. No.: 228 002

Amount: 200 µl

Price: $360.00

Cat. No. 228 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 100 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 44 to 63 from rat SV31 (UniProt Id: A9CMA6)
Reactivity	Reacts with: rat (A9CMA6). Other species not tested yet.
Data sheet	Datasheet 228_002

References for SV31 - 228 002

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2.
Waberer L, Henrich E, Peetz O, Morgner N, Dötsch V, Bernhard F, Volknandt W
Journal of neurochemistry (2017) 1402: 280-293. 228 002 WB

Cat. No.: 228 002

Amount: 200 µl

Price: $360.00

ICC

IHC

IHC-P

Background

SV 31 is a synaptic vesicle protein expressed in a subset of GABAergic and glutamatergic nerve terminals. It is a putative transporter that contains 6 predicted transmembrane domains. Its function has not yet been determined.

1 ) Burré J et al., J. Neurochem. (2007)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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