Synaptic Systems - GABA-A receptor gamma2

Cat. No.: 224 006

Amount: 50 µg

Price: $385.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 224 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity	Reacts with: mouse (P22723), rat (P18508). Weaker signal: zebrafish (E7FF94). Other species not tested yet.
Specificity	Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.
Matching control protein/peptide	224-0P
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE.
Data sheet	Datasheet 224_006

References for GABA-A receptor gamma2 - 224 006

Inhibitory control in neuronal networks relies on the extracellular matrix integrity.
Dzyubenko E, Fleischer M, Manrique-Castano D, Borbor M, Kleinschnitz C, Faissner A, Hermann DM
Cellular and molecular life sciences : CMLS (2021) 7814: 5647-5663. 224 006 WB, IHC; tested species: mouse

Developmental effects of oxytocin on GABAergic neurons in the olfactory brain regions.
Mihalj D, Bukatova S, Reichova A, Havranek T, Bacova Z, Szeiffova Bacova B, Bakos J
Neuroscience (2024) 555: 184-193. 224 006 IHC; tested species: rat

Shank3 Deficiency Results in a Reduction in GABAergic Postsynaptic Puncta in the Olfactory Brain Areas.
Mihalj D, Borbelyova V, Pirnik Z, Bacova Z, Ostatnikova D, Bakos J
Neurochemical research (2024) : . 224 006 IHC; tested species: mouse

Cat. No.: 224 006

Amount: 50 µg

Price: $385.00

ICC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
2 ) Viltono L et al., J. Comp. Neurol. (2008)
3 ) Kralic JE et al., J. Comp. Neurol. (2006)
4 ) Essrich C et al., Nat. Neurosci. (1998)
5 ) Fritschy JM et al., J. Comp. Neurol. (1995)
6 ) Benke D et al., J. Biol. Chem. (1994)
7 ) Fritschy JM et al., Proc. Natl. Acad. Sci. U.S.A. (1992)

Protocols

ICC

Fixed Cells

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GABA-A receptor gamma2 antibody extracellular - 224 006

References for GABA-A receptor gamma2 - 224 006