CtBP1 antibody - 222 002

CtBP1 is a transcriptional corepressor

Rabbit polyclonal antiserum

Cat. No.: 222 002

Amount: 200 µl

Price: $360.00

Cat. No. 222 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 5000 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 414 to 430 from rat CtBP1 (UniProt Id: Q9Z2F5)
Reactivity	Reacts with: rat (Q9Z2F5), mouse (O88712). Other species not tested yet.
Matching control protein/peptide	222-0P
Data sheet	Datasheet 222_002

Cat. No.: 222 002

Amount: 200 µl

Price: $360.00

ICC

Background

CtBP 1 and its close relative CtBP 2 interact with various transcription factors through a PLDLSL motif enhancing transcriptional repression. While CtBP 2 shows a restricted expression pattern CtBP 1 is abundantly expressed in the retina and the brain, where it locates to the nucleus and synaptic nerve terminals.

1 ) Winklmeier A et al., BMC Cancer (2009)
2 ) Kuppuswamy M et al., Mol. Cell. Biol. (2008)
3 ) Corda D et al., Trends Cell Biol. (2006)
4 ) Katsanis N et al., Genomics (1998)

Protocols

ICC

Fixed Cells

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