Tenascin-R antibody - 217 308 K.O.

Tenascin-R is a multidomain adhesion molecule

Guinea pig monoclonal recombinant IgG

Cat. No.: 217 308

Amount: 50 µg

Price: $420.00

Cat. No. 217 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp619
Subtype	IgG2 (κ light chain)
Immunogen	Full length bovine Tenascin-R isolated from brain (UniProt Id: E1BKN2)
Reactivity	Reacts with: rat (Q05546), mouse (Q8BYI9), cow, chicken. Other species not tested yet.
Specificity	K.O. validated
Remarks	This antibody is a chimeric antibody based on the well-known monoclonal mouse antibody 619. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. The antibody can therefore be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Datasheet 217_308

Cat. No.: 217 308

Amount: 50 µg

Price: $420.00

Immunofluorescence image of PFA fixed mouse midbrain section stained for Tenascin-R (red), BCAS1 (green) and NeuN (turquoise)

ICC
(2 img)

IHC
(5 img)

Background

Tenascin-R, also referred to as TNR and J1-160/180, is an extracellular matrix glycoprotein expressed by oligodendrocytes and subpopulations of neurons in the adult CNS of vertebrates. TNR is a member of the Tenascin family of multidomain adhesion molecules and affects neuronal cell migration and neurite extension.

1 ) Zacharias U et al., J. Cell. Sci. (2006)
2 ) Liao H et al., J. Biol. Chem. (2005)
3 ) Milev P et al., J. Biol. Chem. (1998)
4 ) Xiao ZC et al., J. Biol. Chem. (1997)
5 ) Pesheva P et al., J. Neurosci. (1997)

Protocols

ICC

Fixed Cells

IHC

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