Exportin4 antibody - 215 011 K.O.

Exportin4 or XPO4 is a member of the importin β family

Mouse monoclonal purified IgG

Cat. No.: 215 011

Amount: 100 µg

Price: $420.00

Cat. No. 215 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (ECL detection) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P (FFPE): not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	183B1
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 927 to 1151 from human Exportin4 (UniProt Id: Q9C0E2)
Reactivity	Reacts with: human (Q9C0E2), rat, mouse (Q9ESJ0), monkey, ape. Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 215_011

Cat. No.: 215 011

Amount: 100 µg

Price: $420.00

Background

Exportin 4, also referred to as exp4 and XPO4, is a member of the importin β family comprising importins and exportins. These receptor proteins shuttle between the nucleus and the cytoplasm, interact with nuclear pore complexes and bind cargo molecules. Exportin 4 is highly conserved in higher euykaryotes and has been shown to mediate the nuclear export of eIF-5A (eukaryotic translation initiation factor 5A) and Smad 3.
In a recent screen for tumor suppressors an exportin 4 knock-down led to increased tumorigenesis. In addition re-expression of this protein in the exportin 4 deficient cell-line SK-Hep1 efficiently suppressed proliferation.

1 ) Zender L et al., Cell (2008)
2 ) Kurisaki A et al., Mol. Cell. Biol. (2006)
3 ) Lipowsky G et al., EMBO J. (2000)

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