Calbindin/Calretinin antibody - 214 211

Calbindin and Calretinin are vitamin D-dependent calcium binding proteins

Mouse monoclonal purified IgG

Cat. No.: 214 211

Amount: 100 µg

Price: $420.00

Cat. No. 214 211	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	95C1
Subtype	IgG2b (κ light chain)
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P07171, P47728), mouse (P12658, Q08331), grashopper. No signal: zebrafish. Other species not tested yet.
Specificity	Recognizes both isoforms calbindin D28k and D29k / calretinin.
Matching control protein/peptide	214-1P
Data sheet	Datasheet 214_211

Cat. No.: 214 211

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium

Protocols

ICC

Fixed Cells

IHC

IHC-P

Standard Condition