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Calretinin antibody - 214 111BT

Calretinin is a vitamin D-dependent calcium binding protein
Mouse monoclonal purified IgG
Cat. No.: 214 111BT
Amount: 100 µg
Price: $465.00
Cat. No. 214 111BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Label biotin
Clone 37C9
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
Specificity Specific for calretinin / calbindin D29k.
Matching control protein/peptide 214-1P
Data sheet 214_111bt.pdf
Cat. No.: 214 111BT
Amount: 100 µg
Price: $465.00
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.