Synaptic Systems - Calretinin

Cat. No.: 214 102

Amount: 200 µl

Price: $360.00

Cat. No. 214 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331), chicken, zebrafish, grashopper. Other species not tested yet.
Specificity	K.O. validated PubMed: 30015247
Matching control protein/peptide	214-1P
Remarks	IHC-P: This antibody has been successfully applied and published for this method by customers (see application-specific references). It has not been validated using our standard protocols.
Data sheet	214_102.pdf

References for Calretinin - 214 102

Characterization and potential roles of calretinin in rodent spermatozoa.
Dressen C, Schwaller B, Vegh G, Leleux F, Gall D, Lebrun P, Lybaert P
Cell calcium (2018) 74: 94-101. 214 102 WB, IHC-P; KO verified; tested species: mouse,rat

Dendritic Degeneration and Altered Synaptic Innervation of a Central Auditory Neuron During Age-related Hearing Loss.
Wang M, Zhang C, Lin S, Xie R
Neuroscience (2023) 514: 25-37. 214 102 IHC; tested species: mouse

Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 214 102 IHC; tested species: mouse

Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon.
van Heusden F, Macey-Dare A, Gordon J, Krajeski R, Sharott A, Ellender T
Cell reports (2021) 354: 109041. 214 102 IHC; tested species: mouse

Biased auditory nerve central synaptopathy is associated with age-related hearing loss.
Wang M, Zhang C, Lin S, Wang Y, Seicol BJ, Ariss RW, Xie R
The Journal of physiology (2021) 5996: 1833-1854. 214 102 IHC; tested species: mouse

Lack of APP and APLP2 in GABAergic Forebrain Neurons Impairs Synaptic Plasticity and Cognition.
Mehr A, Hick M, Ludewig S, Müller M, Herrmann U, von Engelhardt J, Wolfer DP, Korte M, Müller UC
Cerebral cortex (New York, N.Y. : 1991) (2020) 307: 4044-4063. 214 102 IHC; tested species: mouse

A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space.
Trouche S, Koren V, Doig NM, Ellender TJ, El-Gaby M, Lopes-Dos-Santos V, Reeve HM, Perestenko PV, Garas FN, Magill PJ, Sharott A, et al.
Cell (2019) : . 214 102 IHC; tested species: mouse

Female Mice with Apolipoprotein E4 Domain Interaction Demonstrated Impairments in Spatial Learning and Memory Performance and Disruption of Hippocampal Cyto-Architecture.
Adeosun SO, Hou X, Shi L, Stockmeier CA, Zheng B, Raffai RL, Weisgraber KH, Mosley TH, Ming Wang J
Neurobiology of learning and memory (2019) : . 214 102 IHC; tested species: mouse

Loss of Mecp2 Causes Atypical Synaptic and Molecular Plasticity of Parvalbumin-Expressing Interneurons Reflecting Rett Syndrome-Like Sensorimotor Defects.
Morello N, Schina R, Pilotto F, Phillips M, Melani R, Plicato O, Pizzorusso T, Pozzo-Miller L, Giustetto M
eNeuro () 55: . 214 102 IHC; tested species: mouse

Cat. No.: 214 102

Amount: 200 µl

Price: $360.00

ICC

IHC

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Calretinin antibody - 214 102 K.O.

References for Calretinin - 214 102