ERD2 antibody - 210 003

ERD2 or KDEL is a receptor involved in retrograde Golgi-to-ER transport

Rabbit polyclonal purified antibody

Cat. No.: 210 003

Amount: 50 µg

Price: $380.00

Cat. No. 210 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 200 gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 192 to 212 from rat ERD2 (UniProt Id: Q569A6)
Reactivity	Reacts with: human (P24390, P33947), rat (Q569A6, Q5U305), mouse (Q99JH8, Q9CQM2), monkey, cow, dog. Other species not tested yet.
Specificity	Recognizes Erd 1 and Erd 2.
Data sheet	Datasheet 210_003

Cat. No.: 210 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Retrograde Golgi-to-ER transport by COP 1 coated vesicles serves the recycling of escaped ER-proteins that carry the C-terminal KDEL signal. The KDEL-receptor Erd 2 binds to this signal and retrieves it to the ER. Once returned the Erd 2-ligand complex dissociates and releases Erd 2 for additional recycling rounds.

1 ) Majoul I et al., Dev. Cell (2001)
2 ) Janson IM et al., Biochem. Biophys. Res. Commun. (1998)
3 ) Majoul I et al., J. Cell Biol. (1998)
4 ) Aoe T et al., EMBO J. (1997)
5 ) Lewis MJ et al., Cell (1990)

Protocols

ICC

Fixed Cells

IHC

Standard Condition