Synaptopodin antibody - 163 011 K.O.

Synaptopodin is an actin associated protein present in renal podocytes and telencephalic dendrites

Mouse monoclonal purified IgG

Cat. No.: 163 011

Amount: 100 µg

Price: $420.00

Cat. No. 163 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	109C1
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the central region of mouse Synaptopodin. (UniProt Id: Q8CC35)
Reactivity	Reacts with: mouse (Q8CC35), rat (Q9Z327), human (Q8N3V7). Other species not tested yet.
Specificity	This antibody detects the renal Synpo-long and the neuronal Synpo-short isofoms but is negativ for the T-variant. K.O. validated
Matching control protein/peptide	163-0P
Remarks	IHC-P: A higher concentration (1 : 200) is recommended for human tissue samples.
Data sheet	Datasheet 163_011

Cat. No.: 163 011

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Synaptopodin is the founding member of a class of proline-rich actin-associated proteins and has been originally identified in podocytes of renal glomeruli (1, 2). In brain it localizes to the post-synaptic density (PSD) and the spine aparatus (1). In humans, three isoforms of synaptopodin with a distinct expression profile have been identified, neuronal Synpo-short, renal Synpo-long and Synpo-T. All three isoforms interact with alpha-actinin and induce alpha-actinin-induced actin filaments (3).
Synaptopodin deficient mice lack the dendritic spine apparatus and exhibit impaired activity-dependent long-term synaptic plasticity (4).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Mundel P et al., J. Cell Biol. (1997)
2 ) Mundel P et al., J. Histochem. Cytochem. (1991)
3 ) Asanuma K et al., J. Clin. Invest. (2005)
4 ) Deller T et al., Proc. Natl. Acad. Sci. U.S.A. (2003)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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