GS15 antibody - 199 003

GS15 is a SNARE protein concentrated in the Golgi apparatus

Rabbit polyclonal purified antibody

Cat. No.: 199 003

Amount: 50 µg

Price: $380.00

Cat. No. 199 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 3 to 86 from mouse GS15 (UniProt Id: O35153)
Reactivity	Reacts with: rat (O35152), mouse (O35153). Other species not tested yet.
Matching control protein/peptide	199-0P
Data sheet	Datasheet 199_003

References for GS15 - 199 003

Chlamydia trachomatis hijacks intra-Golgi COG complex-dependent vesicle trafficking pathway.
Pokrovskaya ID, Szwedo JW, Goodwin A, Lupashina TV, Nagarajan UM, Lupashin VV
Cellular microbiology (2012) 145: 656-68. 199 003 ICC

Cat. No.: 199 003

Amount: 50 µg

Price: $380.00

ICC

Background

GS 15 also referred to as GOS 15 and Bet 1L is a member of the SNARE family of proteins and is related to Bet 1. It has been shown to form a complex with syntaxin 5, GOSR 1/GS 28 and Ykt 6. It is involved in early/recycling endosome (EE/RE) to the trans-Golgi network (TGN) trafficking.

1 ) Tai G et al., Mol. Biol. Cell (2004)
2 ) Volchuk A et al., Mol. Biol. Cell (2004)
3 ) Xu Y et al., Mol. Biol. Cell (2002)
4 ) Xu Y et al., J. Biol. Chem. (1997)

Protocols

ICC

Fixed Cells

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