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PACSIN 1 - 196 003

A dynamin 1 associated protein
Polyclonal rabbit purified antibody
Cat. No.: 196 003
Amount: 50 µg
Price: $450.00
Cat. No. 196 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 293 to 384 from mouse PACSIN1 (UniProt Id: Q61644)
Reactivity Reacts with: rat (Q9Z0W5), mouse (Q61644), human (Q9BY11), zebrafish.
Other species not tested yet.
Specificity Specific for PACSIN 1. K.D. PubMed: 30683896
Matching control protein/peptide 196-0P
Data sheet 196_003.pdf

References for PACSIN 1 - 196 003

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, et al.
Nature communications (2019) 101: 428. 196 003 WB, IHC; KD verified; tested species: human,zebrafish
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, et al.
Nature communications (2019) 101: 428. 196 003 WB, IHC; KD verified; tested species: human,zebrafish
Cat. No.: 196 003
Quantity: 50 µg
Price: $450.00
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, et al.
Nature communications (2019) 101: 428. 196 003 WB, IHC; KD verified; tested species: human,zebrafish
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ, et al.
Nature communications (2019) 101: 428. 196 003 WB, IHC; KD verified; tested species: human,zebrafish
Background

This protein has been described as PACSIN 1 (for PKC and CK2 substrate in neurons) and syndapin 1 (synaptic, dynamin-associated protein I). It is a multidomain phospho-protein that interacts via its SH3 domain with dynamin 1, synaptojanin, synapsin and N-WASP. Since N-WASP is an activator of the Arp2/3 complex, syndapin may link membrane trafficking and the actin cytoskeleton.
Up to now, 3 isoforms have been described. Syndapin 1 shows a neuronal, syndapin 3 a muscular and syndapin 2 an ubiquitous expression profile.