Neurobeachin antibody - 194 013

Neurobeachin is a protein kinase A-anchoring protein

Rabbit polyclonal purified antibody

Cat. No.: 194 013

Amount: 50 µg

Price: $380.00

Cat. No. 194 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP-staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Neurobeachin (UniProt Id: Q9EPN1)
Reactivity	Reacts with: mouse (Q9EPN1), rat. Other species not tested yet.
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE.
Data sheet	Datasheet 194_013

Cat. No.: 194 013

Amount: 50 µg

Price: $380.00

Neurobeachin (red) and Synaptobrevin2 (green) staining in a mouse hippocampus section

ICC

IHC

IHC-P

Background

Neurobeachin, also referred to as NBEA, is a large ~330 kDa brain-specific protein that belongs to the BEACH (beige and Chediak-Higashi) family. The protein has been shown to locate to tubulovesicular neuronal endomembranes near the trans-Golgi synapses, but also throughout the cell. Neurobeachin binds to protein kinase A and after adding GTPγS concentrates at Golgi-near membranes.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Su Y et al., J. Neurosci. (2004)
2 ) Wang X et al., J. Neurosci. (2000)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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