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MAP2 antibody - 188 011BT

MAP2 is a major microtubule associated protein in neurons
Mouse monoclonal purified IgG
Cat. No.: 188 011BT
Amount: 100 µg
Price: $470.00
Cat. No. 188 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 200 gallery  
IHC-P: 1 : 200 gallery  
Label biotin
Clone 198A5
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Epitop Epitop: AA 82 to 96 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity Reacts with: human (P11137), rat (P15146), mouse (P20357).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Data sheet 188_011bt.pdf

References for MAP2 - 188 011BT

Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 011BT IHC
Cat. No.: 188 011BT
Amount: 100 µg
Price: $470.00
Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 011BT IHC
Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.