Cat. No.: 188 002
Amount: 200 µl
Price:
350.00 €
Cat. No. 188 002 | 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: yes gallery IHC-P/FFPE: 1 : 250 up to 1 : 500 gallery |
Immunogen | Recombinant protein corresponding to AA 2 to 314 from human MAP2-4 hu (UniProt Id: P11137-4) |
Reactivity |
Reacts with: human (P11137), rat (P15146), mouse (P20357), chicken. Other species not tested yet. |
Specificity | Specific for MAP 2; recognizes all four isoforms. |
Matching control protein/peptide | 188-0P |
Remarks |
WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. |
Data sheet | 188_002.pdf |
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There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.