GluA1 (AMPA1) antibody - 182 511

GluAs are ionotropic glutamate receptors involved in rapid excitatory neurotransmission

Mouse monoclonal purified IgG

Cat. No.: 182 511

Amount: 100 µg

Price: $420.00

Cat. No. 182 511	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 2000 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-70D11
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to C-terminal residues of rat GluA1 (UniProt Id: P19490)
Reactivity	Reacts with: mouse (P23818), rat (P19490). Other species not tested yet.
Matching control protein/peptide	182-01P
Data sheet	182_511.pdf

Cat. No.: 182 511

Amount: 100 µg

Price: $420.00

Indirect immunostaining of rat hippocampus neurons for GluA1 (red) and MAP2 (green)

ICC

IHC

IHC-P

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KAR). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA1, GluA2, GluA3, and GluA4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Andin J et al., Brain Res. (2007)
4 ) Lee HK et al., Mol. Cell. Neurosci. (2007)
5 ) Pei W et al., J. Biol. Chem. (2007)
6 ) Deng YP et al., J. Chem. Neuroanat. (2007)
7 ) Nagy GG et al., J. Neurosci. (2004)
8 ) Lee HK et al., Cell (2003)
9 ) Lee HK et al., Neuron (1998)
10 ) Hughes TE et al., Vis. Neurosci. (1992)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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