Synaptic Systems

Cat. No.: 179 003

Amount: 50 µg

Price: $460.00

WB

ICC

Background

GOSR 1 also known as GOS 28 and GS 28 is a 28 kDa membrane protein on the surface of the Golgi compartment. It has been shown to serve as a vSNARE in ER to Golgi transport.
In co-immunoprecipitation studies GOSR 1 was identified as a member of a SNARE complex consisting of syntaxin 5, membrin (GS 27 GOSR 2, GOS 27), rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
It has been suggested that theses complexes mediate the fusion of ER-derived vesicles with vesicular tubular clusters (VTC), and the fusion of VTCs to form the cis-Golgi compartment.

1 ) Honda A et al., J. Cell Biol. (2005)
2 ) Volchuk A et al., Mol. Biol. Cell (2004)
3 ) Joglekar AP et al., J. Biol. Chem. (2003)
4 ) Shorter J et al., J. Cell Biol. (2002)
5 ) Xu D et al., J. Biol. Chem. (2000)
6 ) Chao DS et al., J. Cell Biol. (1999)
7 ) Hay JC et al., J. Cell Biol. (1998)
8 ) Rowe T et al., Science (1998)

Protocols

WB

ICC

Fixed Cells

Other products for this target

Cat. No. 179 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 155 from rat GOSR1 (UniProt Id: Q62931)
Reactivity	Reacts with: human (O95249), rat (Q62931), mouse (O88630). Other species not tested yet.
Matching control protein/peptide	179-0P
Data sheet	Datasheet 179_003

GOSR1 antibody - 179 003