Synaptic Systems

Cat. No.: 179 002

Amount: 200 µl

Price: $360.00

WB

ICC

Background

GOSR 1 also known as GOS 28 and GS 28 is a 28 kDa membrane protein on the surface of the Golgi compartment. It has been shown to serve as a vSNARE in ER to Golgi transport.
In co-immunoprecipitation studies GOSR 1 was identified as a member of a SNARE complex consisting of syntaxin 5, membrin (GS 27 GOSR 2, GOS 27), rbet1 and rsec22. A more detailed analysis revealed two subcomplexes within this complex. One contains syntaxin 5 (mainly the shorter 35 kDa variant) and GOSR 1 whereas the other is composed of syntaxin 5 (35 and 42 kDa variant), membrin, rsec22 and rbet1.
It has been suggested that theses complexes mediate the fusion of ER-derived vesicles with vesicular tubular clusters (VTC), and the fusion of VTCs to form the cis-Golgi compartment.

1 ) Honda A et al., J. Cell Biol. (2005)
2 ) Volchuk A et al., Mol. Biol. Cell (2004)
3 ) Joglekar AP et al., J. Biol. Chem. (2003)
4 ) Shorter J et al., J. Cell Biol. (2002)
5 ) Xu D et al., J. Biol. Chem. (2000)
6 ) Chao DS et al., J. Cell Biol. (1999)
7 ) Hay JC et al., J. Cell Biol. (1998)
8 ) Rowe T et al., Science (1998)

Protocols

WB

ICC

Fixed Cells

Other products for this target

Cat. No. 179 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 155 from rat GOSR1 (UniProt Id: Q62931)
Reactivity	Reacts with: human (O95249), rat (Q62931), mouse (O88630). Other species not tested yet.
Matching control protein/peptide	179-0P
Data sheet	Datasheet 179_002

GOSR1 antibody - 179 002