Synaptic Systems

Cat. No.: 173 318

Amount: 50 µg

Price: $420.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Immunofluorescence image of a rat cerebellum section stained for GFAP (astrocytes, red) and IBA1 (microglia, green)

WB
(1 img)

ICC
(1 img)

IHC
(4 img)

IHC-P (FFPE)
(7 img)

Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Triolo D et al., J. Cell. Sci. (2006)
2 ) Shiihara T et al., J. Neurol. Sci. (2004)
3 ) Eng LF et al., Neurochem. Res. (2000)
4 ) Dyer CA et al., J. Neurosci. Res. (2000)
5 ) Valentino KL et al., Brain Res. (1983)
6 ) Palfreyman JW et al., J. Neurol. Sci. (1979)
7 ) Jacque CM et al., J. Neurol. Sci. (1978)

Protocols

WB

ICC

Fixed Cells

IHC

IHC-P (FFPE)

IP

Standard Condition

Other products for this target

Cat. No. 173 318	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (fluorescent detection) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp186C6
Subtype	IgG2 (κ light chain)
Immunogen	Full-length recombinant human GFAP (UniProt Id: P14136)
Epitop	AA 1 to 15 from human GFAP (UniProt Id: P14136)
Reactivity	Reacts with: mouse (P03995), rat (P47819), human (P14136). Other species not tested yet.
Specificity	Specific for GFAP, detects all isoforms K.O. validated
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 186C6. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. RUO: For this product an additional Limited Use Label License (LULL) for internal Research Use Only (RUO) from Life Technologies Corporation applies. The recombinant proteins/antibodies you will receive/have received from Synaptic Systems GmbH (“Materials“) were provided or produced under a license between Synaptic Systems GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies“) through the use of Life Technologies proprietary cell lines and expression products. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit, up to and including pharmacokinetic and toxicology studies required to meet the regulations for filing an IND. IND submissions, first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial purposes or applications of any kind, including, without limitation, manufacturing or quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008, United States.
Data sheet	Data Sheet 173_318 Safety Data Sheet (SDS)

GFAP antibody - 173 318 K.O.