Synaptic Systems - Liprin-alpha3

Cat. No.: 169 102

Amount: 200 µl

Price: $360.00

Cat. No. 169 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 463 to 604 from mouse Liprin-α3 (UniProt Id: P60469)
Reactivity	Reacts with: human (O75145), rat (Q91Z79), mouse (P60469). No signal: zebrafish. Other species not tested yet.
Specificity	Specific for liprin-α 3, no cross reaction to isoforms 1, 2, 4.
Matching control protein/peptide	169-1P
Data sheet	Datasheet 169_102

References for Liprin-alpha3 - 169 102

Presynaptic accumulation of α-synuclein causes synaptopathy and progressive neurodegeneration in Drosophila.
Bridi JC, Bereczki E, Smith SK, Poças GM, Kottler B, Domingos PM, Elliott CJ, Aarsland D, Hirth F
Brain communications (2021) 32: fcab049. 169 102 WB; tested species: human

Presynaptic PTPσ regulates postsynaptic NMDA receptor function through direct adhesion-independent mechanisms.
Kim K, Shin W, Kang M, Lee S, Kim D, Kang R, Jung Y, Cho Y, Yang E, Kim H, Bae YC, et al.
eLife (2020) 9: . 169 102 WB; tested species: mouse

Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 169 102 WB; tested species: mouse

Decreased Anxiety-Related Behaviour but Apparently Unperturbed NUMB Function in Ligand of NUMB Protein-X (LNX) 1/2 Double Knockout Mice.
Lenihan JA, Saha O, Heimer-McGinn V, Cryan JF, Feng G, Young PW
Molecular neurobiology (2017) 5410: 8090-8109. 169 102 WB; tested species: mouse

RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 169 102 WB; tested species: rat

Liprin-α controls stress fiber formation by binding to mDia and regulating its membrane localization.
Sakamoto S, Ishizaki T, Okawa K, Watanabe S, Arakawa T, Watanabe N, Narumiya S
Journal of cell science (2012) 125Pt 1: 108-20. 169 102 WB

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 169 102 WB

Cat. No.: 169 102

Amount: 200 µl

Price: $360.00

RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 169 102 WB; tested species: rat

ICC

Background

The liprin-α/Syd 2 protein family was initially identified as binding partners of the LAR family of receptor protein-tyrosine phosphatases.
Liprin-α proteins are multidomain proteins which are involved in the development of presynaptic active zones. Four isoforms of liprin-α have been described, so far and all of them interact with the RIM binding partners ERC 1b and ERC 2.
All four isoforms of liprin-α have also been identified as members of the MALS complex composed of CASK, Mint1 and Velis. This complex has been reported to be crucial for synaptic vesicle exocytosis.

1 ) Hofmeyer K et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Ko J et al., J. Biol. Chem. (2003)
3 ) Ko J et al., J. Neurosci. (2003)
4 ) Kim S et al., J. Biol. Chem. (2003)
5 ) Serra-Pagès C et al., J. Biol. Chem. (1998)

Protocols

ICC

Fixed Cells

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Liprin-alpha3 antibody - 169 102

References for Liprin-alpha3 - 169 102