Liprin-alpha3 antibody - 169 002

Liprin-alpha3 is involved in the development of presynaptic active zones

Rabbit polyclonal antiserum

Cat. No.: 169 002

Amount: 200 µl

Price: $360.00

Cat. No. 169 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 313 to 461 from mouse Liprin-α3 (UniProt Id: P60469)
Reactivity	Reacts with: human (O75145), rat (Q91Z79), mouse (P60469). No signal: zebrafish. Other species not tested yet.
Specificity	This antibody probably cross reacts to isoforms 1, 2, 4, due to sequence homologies.
Matching control protein/peptide	169-0P
Data sheet	Datasheet 169_002

References for Liprin-alpha3 - 169 002

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 169 002 WB

Cat. No.: 169 002

Amount: 200 µl

Price: $360.00

Background

The liprin-α/Syd 2 protein family was initially identified as binding partners of the LAR family of receptor protein-tyrosine phosphatases.
Liprin-α proteins are multidomain proteins which are involved in the development of presynaptic active zones. Four isoforms of liprin-α have been described, so far and all of them interact with the RIM binding partners ERC 1b and ERC 2.
All four isoforms of liprin-α have also been identified as members of the MALS complex composed of CASK, Mint1 and Velis. This complex has been reported to be crucial for synaptic vesicle exocytosis.

1 ) Hofmeyer K et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Ko J et al., J. Biol. Chem. (2003)
3 ) Ko J et al., J. Neurosci. (2003)
4 ) Kim S et al., J. Biol. Chem. (2003)
5 ) Serra-Pagès C et al., J. Biol. Chem. (1998)

Protocols

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