Cat. No.: 163 011
Amount: 100 µg
Price:
$415.00
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| Cat. No. 163 011 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 109C1 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Recombinant protein corresponding to residues near the central region of mouse Synaptopodin. (UniProt Id: Q8CC35) |
| Reactivity |
Reacts with: mouse (Q8CC35), rat (Q9Z327), human (Q8N3V7). Other species not tested yet. |
| Specificity | This antibody detects the renal Synpo-long and the neuronal Synpo-short isofoms but is negativ for the T-variant. K.O. validated |
| Matching control protein/peptide | 163-0P |
| Remarks |
IHC-P: A higher concentration (1 : 200) is recommended for human tissue samples. |
| Data sheet | 163_011.pdf |
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Synaptopodin is the founding member of a class of proline-rich actin-associated proteins and has been originally identified in podocytes of renal glomeruli (1, 2). In brain it localizes to the post-synaptic density (PSD) and the spine aparatus (1). In humans, three isoforms of synaptopodin with a distinct expression profile have been identified, neuronal Synpo-short, renal Synpo-long and Synpo-T. All three isoforms interact with alpha-actinin and induce alpha-actinin-induced actin filaments (3).
Synaptopodin deficient mice lack the dendritic spine apparatus and exhibit impaired activity-dependent long-term synaptic plasticity (4).