Synaptic Systems

Cat. No.: 162 302

Amount: 200 µl

Price: $360.00

Cat. No. 162 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: external data (see remarks) gallery IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity	Reacts with: rat (Q9JLU4), mouse (Q4ACU6). Other species not tested yet.
Specificity	K.O. validated
Remarks	WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. IHC: This antibody has been successfully applied for this method by our customers using mild fixation (2% PFA at pH 6) according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol. IHC-Fr: 4% formaldehyde/PFA fixation is recommended. EM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Datasheet 162_302

References for Shank3 - 162 302

Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse

SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse

SREBP2 gene therapy targeting striatal astrocytes ameliorates Huntington's disease phenotypes.
Birolini G, Verlengia G, Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V, Taroni F, et al.
Brain : a journal of neurology (2021) 14410: 3175-3190. 162 302 WB; tested species: mouse

Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM

Shank3 is localized in axons and presynaptic specializations of developing hippocampal neurons and involved in the modulation of NMDA receptor levels at axon terminals.
Halbedl S, Schoen M, Feiler MS, Boeckers TM, Schmeisser MJ
Journal of neurochemistry (2016) 1371: 26-32. 162 302 WB, ICC

Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM

Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 WB, ICC

REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 162 302 WB

ZCCHC17 knockdown phenocopies Alzheimer's disease-related loss of synaptic proteins and hyperexcitability.
Cortese GP, Bartosch AMW, Xiao H, Gribkova Y, Lam TG, Argyrousi EK, Sivakumar S, Cardona C, Teich AF
Journal of neuropathology and experimental neurology (2024) : . 162 302 ICC; tested species: rat

Autism-Associated Shank3 Is Essential for Homeostatic Compensation in Rodent V1.
Tatavarty V, Torrado Pacheco A, Groves Kuhnle C, Lin H, Koundinya P, Miska NJ, Hengen KB, Wagner FF, Van Hooser SD, Turrigiano GG
Neuron (2020) 1065: 769-777.e4. 162 302 ICC; tested species: mouse

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 162 302 ICC; tested species: rat

Neuronal activity controls transsynaptic geometry.
Glebov OO, Cox S, Humphreys L, Burrone J
Scientific reports (2016) 6: 22703. 162 302 ICC

The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 162 302 ICC

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 WB, ICC

Differential effectiveness of dietary zinc supplementation with autism-related behaviours in Shank2 knockout mice.
Lee K, Jung Y, Vyas Y, Mills Z, McNamara L, Montgomery JM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2024) 3791906: 20230230. 162 302 IHC; tested species: mouse

Dietary zinc supplementation rescues fear-based learning and synaptic function in the Tbr1+/- mouse model of autism spectrum disorders.
Lee K, Jung Y, Vyas Y, Skelton I, Abraham WC, Hsueh YP, Montgomery JM
Molecular autism (2022) 131: 13. 162 302 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 302 IHC; tested species: rat

Modification of the synaptic cleft under excitatory conditions.
Tao-Cheng JH, Moreira SL, Winters CA, Reese TS, Dosemeci A
Frontiers in synaptic neuroscience (2023) 15: 1239098. 162 302 EM; tested species: mouse

Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons.
Tao-Cheng JH
Molecular brain (2020) 131: 53. 162 302 EM; tested species: rat

Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 162 302 EM; tested species: rat

Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM

Cat. No.: 162 302

Amount: 200 µl

Price: $360.00

Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 WB, ICC

Neuronal activity controls transsynaptic geometry.
Glebov OO, Cox S, Humphreys L, Burrone J
Scientific reports (2016) 6: 22703. 162 302 ICC

The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 162 302 ICC

Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 WB, ICC

Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons.
Tao-Cheng JH
Molecular brain (2020) 131: 53. 162 302 EM; tested species: rat

Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM

ICC

IHC

IHC-Fr

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

Fixed Cells

IHC

see remarks

IHC-Fr

Other products for this target

Shank3 antibody - 162 302 K.O.

References for Shank3 - 162 302