Shank1 (SPANK1) antibody - 162 128

Shanks are multidomain proteins of the postsynaptic density

Rabbit monoclonal recombinant IgG

Cat. No.: 162 128

Amount: 50 µg

Price: $420.00

Cat. No. 162 128	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: not recommended IHC-P (FFPE): 1 : 100 (see remarks) gallery IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb329H10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank1 (UniProt Id: Q9WV48)
Reactivity	Reacts with: rat (Q9WV48), mouse (D3YZU1). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 329H10. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-P (FFPE): This antibody shows filamentous background staining on FFPE rat tissue and is recommended for FFPE mouse tissue only. IHC-Fr: The following fixatives are possible: acetone, 4% formaldehyde/PFA.
Data sheet	Datasheet 162_128

Cat. No.: 162 128

Amount: 50 µg

Price: $420.00

Immunofluorescence image of fresh-frozen mouse hippocampus sections stained for Shank1 (red)

ICC
(2 img)

IHC-P (FFPE)
(2 img)

IHC-Fr
(2 img)

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)

IHC-Fr

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