Shank1/2/3 (SPANK1/2/3) antibody - 162 108

Shanks are multidomain proteins of the postsynaptic density

Rabbit monoclonal recombinant IgG

Cat. No.: 162 108

Amount: 50 µg

Price: $420.00

Cat. No. 162 108	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): 1 : 500 gallery IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb151E6
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1055 to 1616 from rat Shank3 (UniProt Id: Q9JLU4)
Reactivity	Reacts with: mouse (Q4ACU6, D3Y2U1, Q80238), rat (Q9JLU4, Q9WV48, Q9QX74). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 151E6. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-Fr: The following fixatives are possible: acetone, 4% formaldehyde/PFA.
Data sheet	Datasheet 162_108

Cat. No.: 162 108

Amount: 50 µg

Price: $420.00

Shank1/2/3 (red) and Neurofilament L (green) staining in fresh frozen murine hippocampus and cerebellum

ICC
(2 img)

IHC-P (FFPE)
(3 img)

IHC-Fr
(2 img)

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)

IHC-Fr

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