|Cat. No. 160 006||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 1000 gallery
IHC: 1 : 500 (see remarks) gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)|
Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3).
Other species not tested yet.
|Specificity||Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.|
|Matching control protein/peptide||160-0P|
IHC: Fix for 15 min with 4% PFA and 15% picric acid in PBS.
Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.