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Home > Products > 155 002

AP180 antibody - 155 002

AP180 is a synaptic protein involved in clathrin mediated endocytosis
Rabbit polyclonal antiserum
Cat. No.: 155 002
Amount: 200 µl
Price: $355.00
Cat. No. 155 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: not recommended (see remarks)
IHC: not recommended
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Reactivity Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog.
Other species not tested yet.
Specificity Specific for AP 180.
Matching control protein/peptide 155-0P
Remarks

ICC: The affinity-purified antibody (cat. no. 155 003) is recommended.
Data sheet 155_002.pdf

References for AP180 - 155 002

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 155 002 WB; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 155 002 WB; tested species: mouse
Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.
Farsi Z, Gowrisankaran S, Krunic M, Rammner B, Woehler A, Lafer EM, Mim C, Jahn R, Milosevic I
eLife (2018) 7: . 155 002 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 155 002 WB; tested species: mouse
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 155 002
Cat. No.: 155 002
Amount: 200 µl
Price: $355.00
Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 155 002 WB; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 155 002 WB; tested species: mouse
Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.
Farsi Z, Gowrisankaran S, Krunic M, Rammner B, Woehler A, Lafer EM, Mim C, Jahn R, Milosevic I
eLife (2018) 7: . 155 002 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 155 002 WB; tested species: mouse
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 155 002
WB
Background

During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of e.g. amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
AP 180 also known as pp155, NP185, F1-20, and SNAP 91 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats.
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