CSP antibody - 154 004

CSP is a chaperon at the synapse

Guinea pig polyclonal antiserum

Cat. No.: 154 004

Amount: 100 µl

Price: $370.00

Cat. No. 154 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P (FFPE): not tested yet IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Immunogen	Synthetic peptide corresponding to AA 182 to 198 from rat CSP (UniProt Id: P60905)
Reactivity	Reacts with: rat, mouse. Other species not tested yet.
Remarks	IHC-G: The following fixatives are possible: 3% glyoxal, 9% glyoxal.
Data sheet	Datasheet 154_004

Cat. No.: 154 004

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-G

Background

Cysteine String Proteins CSPs are composed of an N-terminal J-domain and a central palmitoylated cysteine string. This post-translational modification shifts the molecular weight of CSP 1 in brain from 23 kDa to 34 kDa and confers membrane targeting of the protein.
CSP has been initially identified as a synaptic vesicle protein which is involved in Ca²⁺ triggered neurotransmitter release. Later CSP was also found on Large Dense Core Vesicles (LDCVs) of pancreatic insulin secretory β-cells, chromaffin cells and adipocytes. It has been shown to interact with SNARE proteins like VAMP 2, VAMP 7 and syntaxin 4.

1 ) Smith GB et al., J. Biol. Chem. (2005)
2 ) Evans GJ et al., Eur. J. Neurosci. (2005)
3 ) Miller LC et al., J. Biol. Chem. (2003)
4 ) Chamberlain LH et al., J. Cell. Sci. (2001)
5 ) Chamberlain LH et al., Biochem. J. (1998)
6 ) Chamberlain LH et al., J. Biol. Chem. (1997)
7 ) Chamberlain LH et al., J. Biol. Chem. (1996)

Protocols

ICC

Fixed Cells

IHC

IHC-G

Standard Condition

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