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Home > Products > 152 411

Ca2+ channel R-type alpha-1E antibody - 152 411 K.O.

Mouse monoclonal purified IgG
Cat. No.: 152 411
Amount: 100 µg
Price: $415.00
Cat. No. 152 411 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Clone 62C10
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type α-1E (Cav2.3) (UniProt Id: Q07652)
Reactivity Reacts with: rat (Q07652), mouse.
Other species not tested yet.
Specificity K.O. validated PubMed: 35045307
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel R-type alpha-1E aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 152_411.pdf

References for Ca2+ channel R-type alpha-1E - 152 411

R-type voltage-gated Ca2+ channels mediate A-type K+ current regulation of synaptic input in hippocampal dendrites.
Murphy JG, Gutzmann JJ, Lin L, Hu J, Petralia RS, Wang YX, Hoffman DA
Cell reports (2022) 383: 110264. 152 411 WB; KO verified; tested species: mouse
Cat. No.: 152 411
Amount: 100 µg
Price: $415.00
R-type voltage-gated Ca2+ channels mediate A-type K+ current regulation of synaptic input in hippocampal dendrites.
Murphy JG, Gutzmann JJ, Lin L, Hu J, Petralia RS, Wang YX, Hoffman DA
Cell reports (2022) 383: 110264. 152 411 WB; KO verified; tested species: mouse
WB
Background
Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1E isoform occurs in VGCCs of the R-type which belongs to the high voltage activated group (hva).
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